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Stem Cell Research - COPD, Diabetes, Heart Disease-Abstracts from Dr. Fernandez Viña

Compilation abstracts which have been approved to be presented at

1) CONGESTIVE OBSTRUCTIVE PULMONARY DISEASE (COPD) AND EMPHYSEMA

IMPROVEMENT OF COPD AND EMPHYSEMA WITH AMINISTRATION OF UNFRACTIONS AUTOLOGOUS BONE MARROW ( WORLD PIONEER EXPERIENCE FROM ARGENTINA)

Author Block Fernandez Vina, Roberto1, Saslvsky, Jorge2, Andrin, Oberdan3, Vrsalovic, Francisco1, Fernandez Vina, Federico1, Camozzi, Liliana3, Ferreira da Silva Lima, Janaina4,
1Stem cels therapy and Interventional Cardiology, Fernandez Vina Foundation Argentina and Maimonides University Buenos Aires Argentina, Buenos Aires, Argentina,
2Stem cels therapy and Interventional Cardiology, Medicine School University of Rosario Argentina, Rosario, Argentina, 3Stem cels therapy and Interventional Cardiology, Fernandez Vina Foundation Argentina & San Nicolas Private Hospital, Buenos Aires, Argentina,
4Stem cels therapy and Interventional Cardiology, Fernandez Vina Foundation Argentina, Buenos Aires, Argentina

Abstract:

Objetives: To present the firsts reports and follows up from Argentina of 33 patients, who carry severe chronic obstructive pulmonary disease Emphysematous kind treated with Unfractions Autologous Bone Marrow, All patients have a smoking history for 30-40 years, quit smoking since 6 years ago. Using oxygen 2 lpm 24/7, Ipratropium, nebulizaded Salbuterol , prednison. Grade III-IV short of breath.

Material and Methods: Laboratory Data: Forced vital capacity: 0.5 l (12%), VEF1: 0,30 l (10%) y 0,42 l (13%) post-bronchiodilator, FEF 25-75: 0,14 l/sec(8%), MVV: 11 l/min(10%); TLC: 2.90 l/min (46%), Diffusion Capacity: 10.2 ml/mmHg/min (64%).The patients were submitted to a procedure to extract 600 ml of bone marrow, that presented: Total cells 9184 x10p6/ ml, Total mononuclears 3218 x10p6/ml, CD34+: 4,8 x10p6/ml, CD34+CD38-: 0,72 x10p6/ml. Using IV infusion, in a rhythm of infusion of 300 ml/h.

Evolution and Results: After 60 days of the procedure all the patients were submitted to clinical and laboratorial controls, which presented: Saturation 95-97% in room air, while exercising, free of oxygen. Dispnea Grade 2, decreased of the required medication to 1 Salbuterol nebulization per day in all cases and 12 of them with 1 doses of Ipratropio per day, All patients decreased the doses of prednisone and finally quit with this medication totally. Laboratory data: Forced Vital Capacity: 1,94 l , VEF1: 0,69 l y 0,88 l, FEV25-79: 0,38 l/sec, MVV: 58 l/min, TLC is normal, Diffusion Capacity is Normal.

Conclusions: The autologous adult stem cell from Unfractions Autologous Bone Marrow implanted in lung improve the pulmonary function in patients at less in lung terminal diseases.

2) HEARTH CONDITIONS – CHRONIC MYOCARDIAL ISCHEMIA AND MYOCARDIAL INFARCTION

ADMINISTRATION OF UNFRACTIONS AUTOLOGOUS BONE MARROW IN CHRONIC CORONARY DISEASE ( ARGENTINA PIONEER EXPERIENCE)

Author Block Fernandez Vina, Roberto J.1, Saslavsky, Jorge2, Andrin, Oberdan3, Vrsalovic, Francisco3, Fernandez Vina, Federico4, Camozzi, Liliana5, Ferreira da Silva Lima, Janaina4, Murad Neto, Stans6, Tuma, Jorge7
1Stem cels therapy and Interventional Cardiology, Fernandez Vina Foundation Argentina and Maimonides University Buenos Aires Argentina, Buenos Aires, Argentina, 2Hematology, Medicine School University of Rosario Argentina, Rosario, Argentina,
3Stem cels therapy and Interventional Cardiology, Fernandez Vina Foundation Argentina & San icolas Private Hospital, Buenos Aires, Argentina,
4Stem cels therapy and Interventional Cardiology, Fernandez Vina Foundation Argentina & San Nicolas Private Hospital, Buenos Aires, Argentina, 5Laboratory Clinic, Fernandez Vina Foundation Argentina, Buenos Aires, Argentina,
6Stem cels therapy and Interventional Cardiology, Instituto de Cardilogia de Post Grado Rio De Janeiro Brasil, Rio de Janeiro, Brazil, 7Stem cels therapy and Interventional Cardiology, La Maison de Sante & San Felipe Clinic Lima, Lima, Peru.

Abstract:

Objetives: Unfractions Autologous Bone Marrow may be used in the treatment of chronic myocardial ischemia and myocardial infarction.

Method: 70 patients with refractory angina or cardiac failure and no possibility of surgical revascularization were included. The age of patients (46 men and 24 women) oscillated between 53 and 71 years old. Electrocardiograms showed MI Sequel in different walls of the both ventricles. Echocardiograms showed extensive Myocardiopathy with Diastoles diameter oscillating form 65 to 73mm and the FEjection oscillating between 32% and 34%. Stress test revealed in 52 patients anterior wall and apical necrosis with severe perinecrótic ischemia; in 29 patients inferior and lateral necrosis; and 63 multiple ischemic areas, Ventriculography revealed increment of EDVolume and ESystole Volume, with FE oscillating between 32% and 36%. The Cell Implant was made by “Retrograde Injection through the Sinus Coronary Vein totally occluded with balloon”. The volume injected was 150cc The average of total mononuclears cells implanted 9 x 10p8.

Follow up: After a period of three month a progressive increase of sectors contractility was observed in the echocardiograms. After 180 days it was observed that FE had improved between 38% and 51%. Scintigraphy controls revealed improvement of the perfusion in 62 patients of the perinecrótic and diffuse ischemic areas, 65 patients were subject to Ventriculography, after 360 days and it was observed that the FE improved up to 48%.

Conclusions: The injection of Unfractions Autologous Bone Marrow has demonstrated improvement of the perfusion, surely through angiogenesis that produced the FE increase, objectived by echocardiograms, and Ventriculography. 92% of the patients, showed an improvement of the contractility of border zone of scars. It was not observed any progression of coronary occlusive disease after a period of 4 year. 45patients have achieved 2 year of evolution and they are asymptomatic or in functional class I-II.

3) DIABETES TYPE 1

REPORT FROM ARGENTINA OF FIRST THREE YEARS FOLLOWS UP OF AUTOLOGOUS STEM CELLS IMPLANT IN DIABETES TYPE 1 (WORLD WIDE PIONEER EXPERIENCE)

Author Block Fernandez Vina, Roberto Fernandez Vina
Foundation Argentina, Buenos Aires, Argentina

Abstract:

Background: The adult stem cells CD34 (+)CD38(-) have the capacity to differentiate in functional cells on endocrine pancreas We did the first implant of ASC in the world in diabetes 1 in 2005.

Objectives: To report the long time performance of Diabetic type 1 patients treated with Stems cells implant.

Method: Following the first three years on cell therapy for diabetes 1, the conclusions are optimistic. In this study we have observed the evolution of 38 Diabetic type 1 patients, 20 male, 18 female, average age 23.06 years old (±20.2). All the patients were under insulin therapy. For the transplantation we harvested bone marrow from iliac crest by aspiration, then the sample was processed using a density gradient separation method, obtained 120ml (±95) of CD34(+) and CD38(-) cells solution. For the implant we made a procedure of catheterization through Spleen Artery. No complications or further events were observed during or after the procedure. The patients were sent to clinical and blood samples control during the 12 months following the implant.

Results:
C-Peptide Basal (ng/ml), Basal 0.39 --- 6 Months 0.54 --- 36 Months 1.95 --- Increment 98.30%,
C-Peptide after meal (ng/ml), Basal 0.46 --- 6 Months 0.56 --- 36 Months 2.54 --- increment 151.63%,
H1c Basal 8.4 --- 6 Months 7.39 --- 36 Months 6,54 --- Decrement 24.58%,
nsulin Basal (MU/ml), Basal 5.09 --- 6 Months 12,25 --- 36 Months 18.66 --- Increment23,25%,
Insulin Doses (IU/day): Basal, 54,96 --- 6 Months 35.8 --- 36 Months 12.60 --- Decrement 65,44%.

Conclusions: the implant of mononuclear CD34+ CD38- ( stem cells) from autologous bone marrow improves pancreatic function in patients with type 1 diabetes, in a safe form and is maintained after 3 years at least.

4) DIABETES TYPE 2

FIRST REPORT FROM ARGENTINA OF FIRST THREE YEARS FOLLOWS UP OF AUTOLOGOUS STEM CELLS IMPLANT IN DIABETES TYPE 2

Author Block Fernandez Vina, Roberto1, Ferreira da Silva Lima, Janaina2, Saslavsky, Jorge3, Andrin, Oberdan1, Vrsalovic, Francisco4, Fernandez Vina, Federico1, Camozzi, Liliana5, Dadamo, Carla6
1Stem Cells Therapy and Interventional Cardiology, Fernandez Vina Foundation Argentina and Maimonides University Buenos Aires Argentina, 3Hemetology, Medicine School University of Rosario Argentina, Rosario, Argentina, San Nicolas Private Hospital, Buenos Aires, Argentina, 5Laboratory of Cells Therapy, Fernandez Vina Foundation Argentina & San Nicolas Private Hospital, Buenos Aires, Argentina,
6Stem Cells Therapy and Interventional Cardiology, San Nicolas Clinic & Hospital, Buenos Aires, Argentina.

Abstract:

Objectives: To evaluate the long time performance of Stem cells implant in pancreas in Diabetes Tipe 2 patients The adults stem cells CD34(+)CD38(-) have demonstrate the capacity to differentiate in functional cells on endocrine pancreas. Method: After three year on cell therapy for diabetes patients the conclusions are optimistic. In this study we observed the evolution of 58 patients Diabetic type 2, 37 male, 21 female, 29- 71 years old. 29 patients were under insulin therapy, and 20 patients using Sulphonilureas + Biguanidas. For the transplant were harvest bone marrow from iliac bone crest by aspiration, thereafter the sample was processed using a density gradient separation method, obtained 120ml (±95) of CD34(+)CD38(-) solution. For the implant we did a procedure of catheterization through Spleen Artery. No complications or further events were observed during or after the procedure. The patients were subject to clinical and blood samples control during the following 36 months following after implant.

Results:
C Peptide (ng/ml), Before Implant 1.18 --- 6 Months 1.17 --- 36 Months 2.19 ---, Increment 48.42%, C-Peptide after meal (ng/ml), Before implant 2.22 --- 6 months 2.95 --- 36 months 4.40 --- Increment: 95.52% (p=0.0036) HBC1: 9.14 basal --- 8.25 at 6 months --- at 36 months 6.35 --- Decrement 21,25% (P=0.003) Insulin Basal (MU/ml 12.33 --- 6 months 15.27 --- 36 months 15.02--- Increment 25.26% Insulin After Meal (MU/ml) 19.11 --- 6 months 15.27 --- 36 months 34.7 --- Increment: 58,75% (P=0.016)Pills per day 2,25 prior implant --- after 36 months 0.33 --- (decrement of use 44.36% ( P= 0.0007) Insulin dose (IU/day) basal 50.59 --- after 36 months 9.55 --- Decrement 89,03% (p=0.037)

Conclusions: the implant of mononuclear CD34+CD38- ( stem cells) from autologous bone marrow improve pancreatic function in patients with type 2 diabetes, in a safe form and is maintained after 3 years at least

 
 
 
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